The outcome of cell signaling depends critically on the precise timing and subcellular localization of the various signals. In our laboratory, we combine cell biology with live cell imaging and biophysical tools capable of supplying the required spatiotemporal resolution.
We use Fluorescence Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging (FLIM) to dynamically follow molecular interactions with nanometer resolution, and techniques like high-performance Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Cross Correlation Spectroscopy (FCCS) to reveal protein-protein associations at a sub-millisecond scale.
