The Electron microscopy facility is available for everybody in the NKI who can benefit from high magnifications in his/her research.
In principle the facility performs all necessary steps in the whole procedure from fixation until preparing the figures for publication.
Xiaohu Guo
Apoferritin resolution 3.4Å.
Xiaohu Guo solved the first structure from our F200 microscope Apoferritin to resolution 3.4Å.
The dataset was manually collected, 120 images Particle picking was done by cryolo and initially 34k particles were picked. After 2D and 3D classification, 20k were used for final reconstruction. Using symmetry 432(cubic), the resolution comes to 3.8Å after refinement and finally lands on 3.4Å after polishing.
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