The applications for this technology are widespread, still growing and are common practice in the institute. Every aspect of the deep sequencing process is supported by the GCF that, as a facility, has a large body of experience and knowledge. The facility has been using Illumina technology from the early onset of the NGS development in 2009 and therefore we are now in a position to support the users with clear advice as to how the experiment should be designed to increase the success rate of the experiment. We are open to suggestions and are flexible in the way users want to have their samples analyzed with regard to sequence depth and numbers of libraries that are pooled in multiplexed experiments.
The facility is set up according to a fee-for-service model where the material and chemistry cost prize is the main factor that determines the fee, this makes our support very affordable compared to external service providers.
Facility users can provide cells or tissues as starting material but we also work with purified RNA or DNA as well as ready to sequence DNA libraries. All the necessary equipment for the preparation of sequence libraries and the associated quality control steps are in house. For some applications, like RNAseq, we run the sample preparation in 96-wells format.