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Phase I/II Study of AXL-Specific Antibody-Drug Conjugate Enapotamab Vedotin in Patients with Advanced Solid Tumors.

Kristoffer Staal Rohrberg ,
Juanita S Lopez ,
Mohammed M Milhem ,
Christian U Blank ,
Irene Reijers ,
Fiona Thistlethwaite ,
Ruth Plummer ,
Sarina A Piha-Paul ,
Pasi A Jänne ,
Elaine Shum ,
Heather M Shaw ,
Philip R Debruyne ,
Cristopher Lao ,
Jean-Francois Baurain ,
Jennifer H Choe ,
Eelke Gort ,
Yujie Zhao ,
Guy Jerusalem ,
Patrick Schöffski ,
Andrew William Chen ,
Eric A Cohen ,
Walter C Mankowski ,
Leonid Roshkovan ,
Sharyn I Katz ,
Despina Kontos ,
Lauren K Brady ,
Mohammed Qutaish ,
Patricia Garrido Castro ,
Nora Pencheva ,
Gaurav Bajaj ,
Yali Fu ,
Kristian Windfeld ,
Panagiota Reiter ,
Maria Jure-Kunkel ,
Brandon W Higgs ,
Katayoun I Amiri ,
Tahamtan Ahmadi ,
Ulf Forssmann ,
Suresh S Ramalingam ,
Ignace Vergote

Abstract

PATIENTS AND METHODS

This study comprised dose-escalation and dose-expansion phases; both phases investigated EnaV once every 3 weeks (Q3W) and EnaV on days 1, 8, and 15 of a 28-day cycle (3Q4W). Primary objectives determined the maximum tolerated dose (dose escalation) and safety (dose expansion). Pharmacokinetic profile, antitumor activity, and AXL expression were also assessed.

CONCLUSIONS

EnaV had an acceptable safety profile; however, because the evaluation of antitumor activity did not show clinically meaningful responses, clinical development of EnaV was discontinued.

RESULTS

During dose escalation, 32 patients received EnaV Q3W; 15 received EnaV 3Q4W. The maximum tolerated dose and recommended phase 2 dose were 2.2 mg/kg in Q3W and 1.0 mg/kg in 3Q4W schedules. In dose expansion, 189 patients received EnaV Q3W; 70 received EnaV 3Q4W. Common adverse events in dose expansion included fatigue, constipation, nausea, decreased appetite, and diarrhea. Overall response rates ranged from 4.5% to 12.5% with Q3W dose schedule and from 9.1% to 11.5% with 3Q4W dose schedule. Disease control rates for NSCLC cohorts were 40.9% to 50.0%. NSCLC subset analysis demonstrated correlation between radiomics signature and disease control. The relationship between clinical activity and AXL expression was not apparent.

SIGNIFICANCE

EnaV, an anti-AXL human IgG1 and monomethyl auristatin E antibody-drug conjugate, showed single-agent antitumor activity in preclinical models. This phase 1/2 study of EnaV demonstrated a manageable safety profile and antitumor activity in selected tumor types. Further studies exploring alternative targeting modalities, patient selection, and/or combinations are needed.

PURPOSE

AXL, a receptor tyrosine kinase related to oncogenic processes, is aberrantly expressed in various cancers and associated with treatment resistance. Enapotamab vedotin (EnaV), a novel anti-AXL human IgG1 and monomethyl auristatin E antibody-drug conjugate, demonstrated antitumor activity in preclinical models, including non-small cell lung cancer (NSCLC). This phase 1/2 study assessed the safety and preliminary efficacy of EnaV in solid tumors.

More about this publication

Cancer research communications

Volume 5
Issue nr. 11
Pages 2066-2078
Publication date 01-11-2025

Full text links

Publisher website (DOI) 10.1158/2767-9764.CRC-25-0359
Europe PubMed Central 41166388
Pubmed 41166388

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