Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).
Andrea Cossarizza,
Hyun-Dong Chang,
Andreas Radbruch,
Sergio Abrignani,
Richard Addo,
Mübeccel Akdis,
Immanuel Andrä,
Francesco Andreata,
Francesco Annunziato,
Eduardo Arranz,
Petra Bacher,
Sudipto Bari,
Vincenzo Barnaba,
Joana Barros-Martins,
Dirk Baumjohann,
Cristian G Beccaria,
David Bernardo,
Dominic A Boardman,
Jessica Borger,
Chotima Böttcher,
Leonie Brockmann,
Marie Burns,
Dirk H Busch,
Garth Cameron,
Ilenia Cammarata,
Antonino Cassotta,
Yinshui Chang,
Fernando Gabriel Chirdo,
Eleni Christakou,
Luka Čičin-Šain,
Laura Cook,
Alexandra J Corbett,
Rebecca Cornelis,
Lorenzo Cosmi,
Martin S Davey,
Sara De Biasi,
Gabriele De Simone,
Genny Del Zotto,
Michael Delacher,
Francesca Di Rosa,
James Di Santo,
Andreas Diefenbach,
Jun Dong,
Thomas Dörner,
Regine J Dress,
Charles-Antoine Dutertre,
Sidonia B G Eckle,
Pascale Eede,
Maximilien Evrard,
Christine S Falk,
Markus Feuerer,
Simon Fillatreau,
Aida Fiz-Lopez,
Marie Follo,
Gemma A Foulds,
Julia Fröbel,
Nicola Gagliani,
Giovanni Galletti,
Anastasia Gangaev,
Natalio Garbi,
José Antonio Garrote,
Jens Geginat,
Nicholas A Gherardin,
Lara Gibellini,
Florent Ginhoux,
Dale I Godfrey,
Paola Gruarin,
Claudia Haftmann,
Leo Hansmann,
Christopher M Harpur,
Adrian C Hayday,
Guido Heine,
Daniela Carolina Hernández,
Martin Herrmann,
Oliver Hoelsken,
Qing Huang,
Samuel Huber,
Johanna E Huber,
Jochen Huehn,
Michael Hundemer,
William Y K Hwang,
Matteo Iannacone,
Sabine M Ivison,
Hans-Martin Jäck,
Peter K Jani,
Baerbel Keller,
Nina Kessler,
Steven Ketelaars,
Laura Knop,
Jasmin Knopf,
Hui-Fern Koay,
Katja Kobow,
Katharina Kriegsmann,
H Kristyanto,
Andreas Krueger,
Jenny F Kuehne,
Heike Kunze-Schumacher,
Pia Kvistborg,
Immanuel Kwok,
Daniela Latorre,
Daniel Lenz,
Megan K Levings,
Andreia C Lino,
Francesco Liotta,
Heather M Long,
Enrico Lugli,
Katherine N MacDonald,
Laura Maggi,
Mala K Maini,
Florian Mair,
Calin Manta,
Rudolf Armin Manz,
Mir-Farzin Mashreghi,
Alessio Mazzoni,
James McCluskey,
Henrik E Mei,
Fritz Melchers,
Susanne Melzer,
Dirk Mielenz,
Leticia Monin,
Lorenzo Moretta,
Gabriele Multhoff,
Luis Enrique Muñoz,
Miguel Muñoz-Ruiz,
Franziska Muscate,
Ambra Natalini,
Katrin Neumann,
Lai Guan Ng,
Antonia Niedobitek,
Jana Niemz,
Larissa Nogueira Almeida,
Samuele Notarbartolo,
Lennard Ostendorf,
Laura J Pallett,
Amit A Patel,
Gulce Itir Percin,
Giovanna Peruzzi,
Marcello Pinti,
A Graham Pockley,
Katharina Pracht,
Immo Prinz,
Irma Pujol-Autonell,
Nadia Pulvirenti,
Linda Quatrini,
Kylie M Quinn,
Helena Radbruch,
Hefin Rhys,
Maria B Rodrigo,
Chiara Romagnani,
Carina Saggau,
Shimon Sakaguchi,
Federica Sallusto,
Lieke Sanderink,
Inga Sandrock,
Christine Schauer,
Alexander Scheffold,
Hans U Scherer,
Matthias Schiemann,
Frank A Schildberg,
Kilian Schober,
Janina Schoen,
Wolfgang Schuh,
Thomas Schüler,
Axel R Schulz,
Sebastian Schulz,
Julia Schulze,
Sonia Simonetti,
Jeeshan Singh,
Katarzyna M Sitnik,
Regina Stark,
Sarah Starossom,
Christina Stehle,
Franziska Szelinski,
Leonard Tan,
Attila Tarnok,
Julia Tornack,
Timothy I M Tree,
Jasper J P van Beek,
Willem van de Veen,
Klaas van Gisbergen,
Chiara Vasco,
Nikita A Verheyden,
Anouk von Borstel,
Kirsten A Ward-Hartstonge,
Klaus Warnatz,
Claudia Waskow,
Annika Wiedemann,
Anneke Wilharm,
James Wing,
Oliver Wirz,
Jens Wittner,
Jennie H M Yang,
Juhao Yang
Abstract
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.