Evidence that the 5p12 Variant rs10941679 Confers Susceptibility to Estrogen-Receptor-Positive Breast Cancer through FGF10 and MRPS30 Regulation.
Maya Ghoussaini,
Juliet D French,
Kyriaki Michailidou,
Silje Nord,
Jonathan Beesley,
Sander Canisus,
Kristine M Hillman,
Susanne Kaufmann,
Haran Sivakumaran,
Mahdi Moradi Marjaneh,
Jason S Lee,
Joe Dennis,
Manjeet K Bolla,
Qin Wang,
Ed Dicks,
Roger L Milne,
John L Hopper,
Melissa C Southey,
Marjanka K Schmidt,
Annegien Broeks,
Kenneth Muir,
Artitaya Lophatananon,
Peter A Fasching,
Matthias W Beckmann,
Olivia Fletcher,
Nichola Johnson,
Elinor J Sawyer,
Ian Tomlinson,
Barbara Burwinkel,
Frederik Marme,
Pascal Guénel,
Thérèse Truong,
Stig E Bojesen,
Henrik Flyger,
Javier Benitez,
Anna González-Neira,
M Rosario Alonso,
Guillermo Pita,
Susan L Neuhausen,
Hoda Anton-Culver,
Hermann Brenner,
Volker Arndt,
Alfons Meindl,
Rita K Schmutzler,
Hiltrud Brauch,
Ute Hamann,
Daniel C Tessier,
Daniel Vincent,
Heli Nevanlinna,
Sofia Khan,
Keitaro Matsuo,
Hidemi Ito,
Thilo Dörk,
Natalia V Bogdanova,
Annika Lindblom,
Sara Margolin,
Arto Mannermaa,
Veli-Matti Kosma,
,
Anna H Wu,
David Van Den Berg,
Diether Lambrechts,
Giuseppe Floris,
Jenny Chang-Claude,
Anja Rudolph,
Paolo Radice,
Monica Barile,
Fergus J Couch,
Emily Hallberg,
Graham G Giles,
Christopher A Haiman,
Loic Le Marchand,
Mark S Goldberg,
Soo H Teo,
Cheng Har Yip,
Anne-Lise Borresen-Dale,
,
Wei Zheng,
Qiuyin Cai,
Robert Winqvist,
Katri Pylkäs,
Irene L Andrulis,
Peter Devilee,
Rob A E M Tollenaar,
Montserrat García-Closas,
Jonine Figueroa,
Per Hall,
Kamila Czene,
Judith S Brand,
Hatef Darabi,
Mikael Eriksson,
Maartje J Hooning,
Linetta B Koppert,
Jingmei Li,
Xiao-Ou Shu,
Ying Zheng,
Angela Cox,
Simon S Cross,
Mitul Shah,
Valerie Rhenius,
Ji-Yeob Choi,
Daehee Kang,
Mikael Hartman,
Kee Seng Chia,
Maria Kabisch,
Diana Torres,
Craig Luccarini,
Don M Conroy,
Anna Jakubowska,
Jan Lubinski,
Suleeporn Sangrajrang,
Paul Brennan,
Curtis Olswold,
Susan Slager,
Chen-Yang Shen,
Ming-Feng Hou,
Anthony Swerdlow,
Minouk J Schoemaker,
Jacques Simard,
Paul D P Pharoah,
Vessela Kristensen,
Georgia Chenevix-Trench,
Douglas F Easton,
Alison M Dunning,
Stacey L Edwards
Abstract
Genome-wide association studies (GWASs) have revealed increased breast cancer risk associated with multiple genetic variants at 5p12. Here, we report the fine mapping of this locus using data from 104,660 subjects from 50 case-control studies in the Breast Cancer Association Consortium (BCAC). With data for 3,365 genotyped and imputed SNPs across a 1 Mb region (positions 44,394,495-45,364,167; NCBI build 37), we found evidence for at least three independent signals: the strongest signal, consisting of a single SNP rs10941679, was associated with risk of estrogen-receptor-positive (ER+) breast cancer (per-g allele OR ER+ = 1.15; 95% CI 1.13-1.18; p = 8.35 × 10-30). After adjustment for rs10941679, we detected signal 2, consisting of 38 SNPs more strongly associated with ER-negative (ER-) breast cancer (lead SNP rs6864776: per-a allele OR ER- = 1.10; 95% CI 1.05-1.14; p conditional = 1.44 × 10-12), and a single signal 3 SNP (rs200229088: per-t allele OR ER+ = 1.12; 95% CI 1.09-1.15; p conditional = 1.12 × 10-05). Expression quantitative trait locus analysis in normal breast tissues and breast tumors showed that the g (risk) allele of rs10941679 was associated with increased expression of FGF10 and MRPS30. Functional assays demonstrated that SNP rs10941679 maps to an enhancer element that physically interacts with the FGF10 and MRPS30 promoter regions in breast cancer cell lines. FGF10 is an oncogene that binds to FGFR2 and is overexpressed in ∼10% of human breast cancers, whereas MRPS30 plays a key role in apoptosis. These data suggest that the strongest signal of association at 5p12 is mediated through coordinated activation of FGF10 and MRPS30, two candidate genes for breast cancer pathogenesis.