Clinical applicability of the Polygenic Risk Score for breast cancer risk prediction in familial cases.

We included 3918 BC cases from 3492 Dutch non-BRCA1/2 BC families and 3474 Dutch population controls. The association of the standardised PRS₃₁₃ with BC was estimated using a logistic regression model, adjusted for pedigree-based family history. Family history of the controls was imputed for this analysis. SEs were corrected to account for relatedness of individuals. Using the BOADICEA (Breast and Ovarian Analysis of Disease Incidence and Carrier Estimation Algorithm) V.5 model, lifetime risks were retrospectively calculated with and without individual PRS₃₁₃. For 2586 cases and 2584 controls, the carrier status of pathogenic variants (PVs) in ATM, CHEK2 and PALB2 was known.

Our results support the application of the PRS₃₁₃ in risk prediction for genetically uninformative BC families and families with a PV in moderate BC risk genes.

The family history-adjusted PRS₃₁₃ was significantly associated with BC (per SD OR=1.97, 95% CI 1.84 to 2.11). Including the PRS₃₁₃ in BOADICEA family-based risk prediction would have changed screening recommendations in up to 27%, 36% and 34% of cases according to BC screening guidelines from the USA, UK and the Netherlands (National Comprehensive Cancer Network, National Institute for Health and Care Excellence, and Netherlands Comprehensive Cancer Organisation), respectively. For the population controls, without information on family history, this was up to 39%, 44% and 58%, respectively. Among carriers of PVs in known moderate BC susceptibility genes, the PRS₃₁₃ had the largest impact for CHEK2 and ATM.

Common low-risk variants are presently not used to guide clinical management of familial breast cancer (BC). We explored the additive impact of a 313-variant-based Polygenic Risk Score (PRS₃₁₃) relative to standard gene testing in non-BRCA1/2 Dutch BC families.