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Risk stratification and relapse pattern in triple-negative breast cancer with pathological complete response after neoadjuvant treatment: the European GAMBIT real-world study.

Davide Massa ,
Theodoros Foukakis ,
Sylvie Giacchetti ,
Christine Desmedt ,
Jean-Sebastien Frenel ,
Elisa Gasparini ,
Marleen Kok ,
Paolo Vigneri ,
William Jacot ,
Claudio Vernieri ,
Matteo Lambertini ,
Francesca Patanè ,
Federico Piacentini ,
Emilio Bria ,
Alberto Zambelli ,
Dario Trapani ,
Andrea Botticelli ,
Ornella Garrone ,
Stefania Lando ,
Alexios Matikas ,
Laetitia Someil ,
Giuseppe Floris ,
Mario Campone ,
Moira Ragazzi ,
Esther Lips ,
Federica Martorana ,
Giorgio Bonomi ,
Andri Papakonstantinou ,
Lorenzo Nicolè ,
Ioannis Zerdes ,
Patrick Neven ,
Martina Rotolo ,
Hugo Horlings ,
Sabrina Nucera ,
Amélie Gudin-De-Vallerin ,
Andrea Vingiani ,
Angelo Paolo Dei Tos ,
Valentina Guarneri ,
Maria Vittoria Dieci ,

Abstract

Pathologic complete response after neoadjuvant treatment is considered a surrogate of cure in triple-negative breast cancer, yet around 10% of patients still relapse. Whether baseline stromal tumor-infiltrating lymphocytes can stratify this residual risk is unknown. Here, we report on GAMBIT, a multicentric real-world retrospective study of 2457 patients with triple-negative breast cancer or estrogen receptor-low disease, HER2-negative, of whom 1192 obtained a pathological complete response and 690 have evaluable tumor infiltrating lymphocytes. Among patients with pathological complete response, clinical nodal status and tumor infiltrating lymphocytes are independently prognostic and patients with clinical node-positive/low-tumor infiltrating lymphocytes tumors experience substantially worse outcomes, with five-year distant relapse-free survival of 83.4% and overall survival of 85.8%. In this high-risk subgroup, five-year cumulative incidence of central nervous system reaches 7.5%, including 6.9% presenting as isolated central nervous system relapse. In this work, we identify a high-risk subgroup despite pathologic complete response and provide a framework supporting risk-adapted trial design incorporating central nervous system-directed strategies.

More about this publication

Nature communications

Publication date 24-06-2026

Full text links

Publisher website (DOI) 10.1038/s41467-026-74056-2
Europe PubMed Central 42343076
Pubmed 42343076

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