Generation of mixed murine organoids to model cellular interactions.


Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).

More about this publication

STAR protocols
  • Volume 2
  • Issue nr. 4
  • Pages 100997
  • Publication date 17-12-2021

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