Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform.


Functional genomic screens using shRNA technology are a great tool in biomedical research. As more labs gain access to the necessary reagents and technology to perform such screens, some may lack in-depth knowledge on the difficulties often encountered. With this protocol, we aim to point out the most important caveats of performing shRNA based screens and provide a streamlined workflow that can be easily adapted to meet the specific needs of any particular screening project.

More about this publication

Methods in molecular biology (Clifton, N.J.)
  • Volume 1470
  • Pages 49-73
  • Publication date 02-09-2016

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