Protocol for live-cell imaging during Tumor Treating Fields treatment with Inovitro Live.


Tumor Treating Fields (TTFields) are an FDA-approved anticancer treatment using alternating electric fields. Here, we present a protocol to perform live-cell imaging (LCI) of cells during TTFields treatment with the Inovitro LiveTM system. The setup we describe dissipates TTFields-related heat production and can be used in conjunction with any LCI-compatible microscope setup. This approach will enable further elucidation of TTFields' mechanism of action at the molecular level and facilitate the development of promising combination strategies.

More about this publication

STAR protocols
  • Volume 3
  • Issue nr. 2
  • Pages 101246
  • Publication date 17-06-2022

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