CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes.


High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-interference (CRISPRi), with a traditional short hairpin RNA (shRNA)-based system for performing lethality screens. We find that the CRISPR technology performed best, with low noise, minimal off-target effects and consistent activity across reagents.

More about this publication

Nature biotechnology
  • Volume 34
  • Issue nr. 6
  • Pages 631-3
  • Publication date 01-06-2016

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