Rob van der Kammen

Technician

Beginning may 2025, I joined the Brummelkamp lab as a Technician. Together with Gian-Luca we will explore the role of DIESL in cell fitness, metabolism and cancer.

Biochemistry

Cell Biology

Molecular biology

Work experience

- Improving the organisation by setting up databases for plasmids, primers, antibodies etc.

- Breeding mouse lines, crossing with other genetically modified mice and subsequent genotyping. Controlling mice and experiments via Promas database.

- Anatomical section on ill mice, removing tumors,SC/ IP injection

- Isolation of keratinocytes and mefs from mice.

- Molecular biology; Northen/Southern blotting, DNA/RNA isolations cloning, RT-PCR, ShRNA screening, competent cells( making of), Isolation of eco/amphotropic virusses, Lenti and Adeno.

- Culturing cells from epithelial, fibroblastic, neuronal and lymphoid origin,

- Isolation of proteins from bacteria (especially big proteins)

- Making antibodies

- Westernblot, IP’s. Mass spec analysis.

- RNA seq/Qpcr

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