Work experience
- Improving the organisation by setting up databases for plasmids, primers, antibodies etc.
- Breeding mouse lines, crossing with other genetically modified mice and subsequent genotyping. Controlling mice and experiments via Promas database.
- Anatomical section on ill mice, removing tumors,SC/ IP injection
- Isolation of keratinocytes and mefs from mice.
- Molecular biology; Northen/Southern blotting, DNA/RNA isolations cloning, RT-PCR, ShRNA screening, competent cells( making of), Isolation of eco/amphotropic virusses, Lenti and Adeno.
- Culturing cells from epithelial, fibroblastic, neuronal and lymphoid origin,
- Isolation of proteins from bacteria (especially big proteins)
- Making antibodies
- Westernblot, IP’s. Mass spec analysis.
- RNA seq/Qpcr