We established the role of combined inactivation of E-cadherin and PTEN in a genetically engineered mouse model of invasive lobular breast cancer. In (A), H&E and Masson’s trichrome staining (revealing collagen) and IHC of PDGFRβ (fibroblasts), CD3 (T cells), and F4/80 (macrophages) in tumor-free mammary glands and CLCs from wild-type, Wcre;Cdh1F/F, Wcre;PtenF/F, and Wcre;Cdh1F/F;PtenF/F mice of indicated age were used for histopathologic classification of tumors and to examine stromal compositions in each indicated genotype, which were subsequently compared to (B) the microenvironment of healthy human mammary glands and the tumor microenvironment of human CLCs.
Boelens et al., Cell Reports, 2016, PMID: 27524621
