We work together with scientists on bio-imaging projects and provide support for image acquisition and analysis using advanced microscopy and software tools. The facility is a place where scientists meet, work together on bio-imaging projects and receive support by experts for image acquisition and analysis using advanced microscopy and software tools.
- Zeiss LSM 980 confocal with Airyscan2
A point-scanning system on an inverted Observer Imager Z2 microscope with a motorized stage and a variety of objectives (air/oil/water/glycerine), also suitable for DIC. Available laser lines are: 405, 442, 458, 488, 514, 561, 594 and 633 nm. The system has two standard PMT detectors (tuneable), a more sensitive GaAsP spectral detector array and an Airyscan2 detector with fast mode.
- Leica TCS SP8 confocal
This point-scanning system is based around a fully motorized Leica DM8 inverted microscope equipped with a variety of objectives (air/oil/water) also suitable for DIC. Fluorescence detection is tuneable (400-800nm) with five internal detectors (3x HyD and 2x PMT). Available laser lines are 405, 442, 458, 476, 488, 514, 561, 594 and 633 nm. The scan head has two scanners: conventional (standard) and resonant (fast dynamic). Software licenses for online adaptive deconvolution (Lightning) and Navigator for easy imaging of large regions.
- Leica SP5 confocal (2x)
A point-scanning system on an inverted DM6000 microscope with fully motorized stage and a variety of objectives (air/oil/water), also suitable for DIC. It has five tuneable fluorescence detectors (3x HyD and 2x PMT). Available laser lines are 405, (442), 458, 476, 488, (496), 514, 561, 594, 633 nm. Incubation chamber for CO2 and temperature-control is present on one of the systems. This system is also equipped with a resonant scanner for fast dynamic imaging.
- Andor Dragonfly spinning disk confocal
A spinning disk microscope on an inverted Leica DMI8 microscope with an incubation chamber for temperature and CO2 control. 2 Andor Zyla 4+ sCMOS cameras can be used simultaneously. Capable of bright field, DIC, multi-color fluorescence, z-series, time lapse and multi-stage position imaging. Seven excitation lasers (405, 442, 488, 514, 561, 594 and 637nm). Equipped with TIRF module.
- Zeiss Axio Observer Z1 Live (2x)
High-quality, inverted, wide field microscope systems with motorized stage control (for z sectioning, time lapse, tiling), autofocus and temperature and CO2 control for live cell imaging. The systems are equipped with sensitive Hamamatsu Orca Flash 4 monochrome cameras for bright field, DIC, phase contrast and fluorescence imaging (LED or HXP light source). Standard filter cubes are installed (DAPI/CFP/YFP/GFP/mCherry/CY5), more filtersets available on request.
- Zeiss Axio Imager Z1 Fixed
The fully motorized Zeiss AxioObserver Z1 inverted microscope is equipped with a sensitive Hamamatsu Orca Flash 4 monochrome camera for brightfield and phase contrast imaging. Stage inserts allow imaging of cells on glass slides, multi well plates or dishes up to 10cm. Filter cubes currently installed on the microscope are for DAPI, Alexa488, Alexa568, Cy5 and triple filter cubes excellent for FISH experiments (incl. Blue/SpectumGreen/SpectrumOrange). More filters are available on request. Possibility for multi position, Z-stacks and mosaic tiled images.
- Zeiss Axiovert 200M Color
This wide field microscope is suited for high resolution imaging of (immuno-)histochemically stained tissue sections and cells. The system is equipped with a Zeiss high resolution AxioCam 512 color camera and a motorized x/y scanning stage. Objectives are available in the range of 1.25x to 100x. Stage inserts allow imaging of cells on glass slides, multi well plates or dishes up to 10cm. Possibility to create mosaic images from large tissue samples.
- Zeiss Axio Scan.Z1 - Fluorescence Slide Scanner
The AxioScan is an automated slide scanner for brightfield and fluorescence microscopy. Up to 100 slides can be loaded and scanned with 10x/0.45, 20x/0.8 or 40x/0.95 objectives. The system creates high-quality virtual slides that can be navigated, zoomed in, etc. Equipped with a sensitive Hamamatsu Orca Flash 4.0 monochrome camera (for fluorencence slides) and a Hitachi HV-F203 color camera (for brightfield). Multiple fluorescence filtersets available.
- Zeiss AxioZoom V16
Combines the advantages of a stereomicroscope - zoom optics and long working distance - with the high resolution of a light microscope (NA 0.25). The motorized zoom allows quick and easy switching through the entire 16x zoom range without changing objectives. The macroscope is equipped with both fluorescent and transmitted light. You can view and image large samples (e.g. a complete well of a 12 well plate) with excellent brightness and resolution without the need for tiling and stitching. In addition, the long working distance allows for dissection of small tissue samples.
- Leica TIRF DMI6
Equipped with four diode lasers: 405, 488, 561 and 635 nm and an ANDOR iXon Ultra 888 EM-CCD camera. Temperature controlled live cell chamber with 5% CO2 if necessary. Besides the specific TIRF equipment the system also contains a complete setup for Phase Contrast- and DIC imaging, with objective magnifications in the range 10x to 100x for time-lapse experiments with multichannel fluorescence, Z-stack, multi position and mosaic imaging.
- HIVE- data analysis and storage server for streaming and processing& analysis of data. The following programs are installed Zeiss ZEN, Leica LASX, Huygens, Python, Qupath, ImageJ/Fiji)Workstations
- Three high-end workstations with image processing & analysis software (Huygens, Imaris, Matlab, Leica LAS X en Zeiss ZEN, Qupath, Cell profiler, FIJI/ImageJ)