Working at the NKI
Find out more about working at the Netherlands Cancer Institute
Throughout the year we have vacancies for group leaders,
postdoctoral fellows, Ph.D. students and technicians. Also, for
masters students and student technicians we offer rotation
projects. Below we have listed the current vacancies. If you are
interested in a position at our institute, please contact one of
our group leaders.
We offer a stimulating and interactive research environment, state of the art facilities, a competitive salary (including possibilities for additional tax-reduction for non-Dutch employees) and housing facilities in the vicinity of the Institute. Researchers at the Netherlands Cancer Institute have no teaching obligations.
On the Education page you can see which vacancies are currently available for Ph.D. students and postdocs.
Vacancy Immunologist for developing melanoma therapies (Postdoc)
Vacancy PhD student Risk Modelling
Vacancy PhD Student (applied) mathematics, industrial engineering or a related field
Vacature (BBL) Proefdierverzorger
- Carcinogen laboratory
- Digital Microscopy facility
- Flow cytometry facility
- Mouse clinic - transgenic core facility
- Radionuclides centre
- Protein facility
- Technology Transfer Office
- Electron microscope facility
- Genomics Core Facility
- Mouse Clinic Intervention unit
- Peptide facility
- Molecular Pathology & Biobanking Core facility
- Biostatistics center
- TTO team members
- Research Collaborations, Materials
- Reagents for licensing
- Pattended technologies for licensing
- News and events
- Grants and Subsidies
- People of the TTO
- Basic Medical Statistics Course: Faculty
- Basic Medical Statistics Course: Course Material
- Supplemental Material
Facts & figures
In cancer research we use a lot of dangerous compounds.
The carcinogen lab is a research facility where carcinogenic,
mutagenic and reprotoxic compounds can be stored and were these
compounds can be handled under special safety conditions.
The carcinogen laboratory is mainly used to prepare solutions using chemicals in a powder form. The chemicals can be weighted in a fume hood, dissolved and then taken out of the carcinogen laboratory for use in experiments. The lab is under-pressured, has a higher than normal ventilation fold and is equipped with two safety hoods.
Researchers can get instructions on how to work with carcinogens and about safety aspects. The instructions and the information is given by one of the supervisors of this facility. They also make sure everything is working properly and is kept clean and tidy.
Click here for more information about the Carcinogen laboratory
Digital Microscopy facility
The Digital microscopy facility offers high level and broad range light microscopy facilities to all researchers in the Netherlands Cancer Institute. The equipment consists of nine microscopes: four confocals, four wide-field ones and a TIRF setup. Together they form a complementary set of instruments. Live cell imaging capabilities are implemented on three confocals, two wide-field microscopes and the TIRF setup. The staff provides extensive support to users by e.g. introducing them to the systems, offering help and advise in using the microscopes and taking care of image archiving. Importantly, continuous quality control of the instruments, in its broadest sense, is provided as well.
Click here for more information about the Digital Microscopy facility
Flow cytometry facility
Flow cytometry is a powerful tool to quickly characterize millions of individual cells and, if needed, sort the cells based on their characteristics. The analysis is based on the size and complexity of the cell, in combination with the presence of fluorescence signals in the form of antibodies, fluorescent proteins or other dyes. The Flow cytometry facility gives researchers access to a variety of cell analysers and sorters. The dedicated operators of the flow cytometry facility give training, technical support with experiment setup, take care of the maintenance of the equipment and perform sorting experiments.
Click here for more information about the Flow cytometry facility.
Mouse clinic - transgenic core facility
The Netherlands Cancer Institute has a very strong history in the generation and validation of mouse models of human cancer. Many conditional mouse strains for tumor suppressor alleles have been generated here and are routinely used worldwide. In that context, the Transgenic core facility accommodates all activities required to generate genetically modified animals as well as cryopreservation.
Click here for more information about the Mouse clinic - transgenic core facility.
The Radionuclides centre (RNC) is the facility for researchers who want to perform their radioactive experiments in the Netherlands Cancer Institute. This centre has several containment levels (B, C and D-level) in which a range of experiments can be performed. Also, the facility provides regular courses (Radiation Protection level 5B) for people who want to use radioactivity during their experiments. The staff of the RNC provides help and advice on various aspects of radioactivity.
Click here for more ionformation about the Radionuclides centre.
The Protein facility of the Netherlands Cancer Insitute provides support at all levels of protein research, including the production and purification of proteins, biophysical characterization and high-throughput protein crystallization screening. The facility offers know-how, (biological) tools and access to dedicated equipment to assist both in routine- as well as more challenging projects.
Click here for more information about the Protein facility.
Technology Transfer Office
The NKI has a mission to beat cancer and performs world-beating
research to increase our understanding of this disease. The
knowledge that is gained in this way sometimes opens avenues for
development of e.g. novel treatments or new diagnostic tests.
To achieve its mission, NKI actively collaborates with private companies that have the knowledge and the means to develop products based on research results obtained at our institution.
The Technology Transfer Office (TTO) of NKI is charged with
providing support for the research base in contracting with other
research organizations and with industry. TTO takes the lead
in negotiations for research-based agreements and monitors the
rights and obligations of NKI under such contracts.
It also evaluates research results for commercial potential and if needed will apply for patent rights and help steer further development of those results towards commercial application.
TTO is actively sourcing interest from commercial parties to help put our knowledge to work in developing novel products that benefit cancer patients. On occasion, a new company is set up to function as a vehicle for investment in such development.
For more information, contact TTO at +31 (0)20 512 1999 or via firstname.lastname@example.org.
Electron microscope facility
The Electron microscope facility enables researchers at the Netherlands Cancer Institute to look at cellular structures and protein complexes at high magnification.
The facility has two electron microscopes. Since 1990 we have a Philips CM10 electron microscope operating with acceleration voltages of 40-60-80-100 kV fitted with a tungsten emitter. This electron microscope still makes use of negatives that are developed and printed by our photographer Nico Ong. Since 2001 we have a FEI Tecnai 12 G2 Biotwin 120 kV microscope fitted with a LaB6 emitter and an Eagle 4Kx4K fiber-optically coupled, TEM CCD camera. This microscope is suited for cryoEM and tomography. It has integrated FEI Explore 3D and Inspect 3D software for aligning and reconstructing tomogram series.
The dedicated EM operator maintains the microscopes and performs the sample preparation and acquisition of data.
Genomics Core Facility
The Genomics Core Facility offers Next Generation Sequencing services using Illumina equipment (HiSeq2000 and MiSeq). Next generation sequencing is a very versatile technology that has applications in many different experiments. Sequencing data can be used to discover mutations in the exome or smaller targeted gene sets, find genome wide copy-number variations, analyze RNA expression levels or read complete inventories of small RNA or the results of functional genetic screens. In addition to the wet lab part the facility also provides data storage and bioinformatics support and maintains access to several commercial tools like Ingenuity and Nexus. Investigators have the option to hand in cells, tissue, tumor, RNA, DNA or prepared sequence libraries for analysis.
Click here for more information about the Genomics Core Facility.
Robotics and Screening Center
Mouse Clinic Intervention unit
Our goal is to use advanced mouse models as surrogate cancer patients to identify and validate targets that can be exploited by anti-cancer therapy. Various approaches to treat cancer with classical chemotherapy, targeted inhibitors, immunotherapy, radiotherapy or combinations thereof are ongoing. Special emphasis is given to target the clinical handicap of therapy escape. To support these activities, a dedicated preclinical intervention and imaging unit facilitates accurate measurement of tumor growth, metastasis formation, and therapy response in several mouse models of human cancer.
Click here for more information about the Mouse Clinic Intervention unit.
Research IT is a facility
The Peptide facility synthesizes peptides for researchers. The
is done on a small scale (2 micromole for screening purposes) and up to
millimole scale. Long peptides up to 100 amino acids (ubiquitine for instance) can be synthesized and all kind of derivatives are possible (biotinylation,
phophorylation, dyes etc.). Quality check and purification of the peptides is
also part of the service.
Click here for more information about the Peptide facility.
Molecular Pathology & Biobanking Core facility
The Core Facility Molecular Pathology & Biobanking (CFMPB) registers, coordinates, assists and facilitates research involving archived human/patient material. This concerns all research using NKI-AVL Biobank material from the department of Pathology (the FFPE tissue archive and frozen tissue bank) and the department of Clinical Chemistry (the serum and blood biobank). The facility provides professional expertise, appropriate sample and tissue based experimentation, and implements optimally controlled medical-ethical issues according to the 'Code of conduct'.
Click here for more information about the Molecular Pathology & Biobanking.
Biostatistics is a key component in planning, conducting and analysing studies in biomedical cancer research, including epidemiology. In order to employ state-of-the-art statistical approaches, investigators and doctors from the Institute and the hospital may contact the Biostatistics Center for statistical advice. We are involved in developing and implementing various methods to cover a wide range of topics including the design and analysis of epidemiologic studies and clinical trials, the identification of prognostic and predictive biomarkers, sample size calculations, risk prediction, as well as animal and in vitro experiments. The Biostatistics Center also conducts the annual one-week Basic Medical Statistics course, attended by more than 50 graduate students.
Click here for more information about the Biostatistics Center.
TTO team members
Effective Technology transfer requires a combination of skills, which is reflected in the backgrounds of the various people from the NKI TTO team:
Manager Technology Transfer Office
holds a PhD in molecular virology and started his career in tech transfer when
he joined the Medical Research Council in 2001 as a technology transfer manager
(through its London-based subsidiary company MRC Technology). Upon return to
the Netherlands, Koen founded the TTO at VU University
in Amsterdam - which later expanded its activities to also include VU
University - where he was responsible for IP and licensing until moving to
NKI-AVL in May 2009. Koen served as a board member of ASTP-Proton, the
European professional association for technology transfer, from 2010 - 2014 and
is a Registered Technology Transfer Professional (RTTP).
Research Collaborations, Materials
Collaborative projects and contract research
Researchers and research groups can set up collaborative research projects ogether with companies or other organizations. They can also carry out services or research contracts at the request of companies or other clients. The TTO of the Netherlands Cancer Institute (NKI) supports researchers in drafting, egotiating and monitoring these agreements.
Material Transfer Agreements
A Material Transfer Agreement (MTA) is an agreement where a party agrees to transfer to another party, under defined conditions, materials (for example a biochemical material - such as nucleic acids, plasmids, peptides, proteins, viruses, cells, tissues samples, living organisms etc.), mostly for use in research and development. MTAs allow us to control the distribution (and sometimes the use) of research materials that were uniquely created at NKI-AVL.
The Technology Transfer Office of NKI is responsible for drafting, egotiating and monitoring all MTAs and must be involved when research materials are to be exchanged. We speak of an "Incoming MTA" or "MTA In" where a research group at NKI receives a material from a third party. An 'Outgoing MTA' or 'MTA Out' stands for an MTA under which a research group at NKI sends out material to a third party. The fact that NKI-AVL handles many more MTA's Out than MTAs In points to its leadership position in the field of cancer research.
To stimulate further research and as a service to the academic community, transfer of research materials to other academic, not-for-profit research organizations by NKI will carry no charge and will be against terms that would normally be acceptable to NKI in the reverse situation. In exceptional cases, a handling fee may be charged where NKI needs to put significant effort into the preparation of the requested material. Shipment costs are always charged to the receiving organization.
Commercial entities requesting the use of research materials will typically be charged a fee for transfer and use of proprietary research materials from NKIL. These fees will support further research within NKI, both for the providing research group as well as for the organization as a whole.
The main provision within a standard MTA prohibits the receiving party from distributing the material any further (sometimes even to other researchers within the same organization). When sending out research materials, TTO needs to check first whether we have the unconstrained right to do so.
Use of tumor tissue in collaborative research
NKI can share patient-derived (tumor) tissue with other organizations for purposes of scientific research in the context of a collaboration with NKI. Requests for materials from outside will be reviewed by the Translational Research Board of the institute. If approved, a Sample Transfer Agreement (in fact a specialized version of a material transfer agreement) will be drawn up for signature by the receiving organization.
Reagents for licensing
- NKI has an exclusive production/distribution agreement for many of its monoclonal antibodies with Nordic/Mubio.
- NKI does not license its hybridoma cell lines. Interested companies can purchase high quality purified antibodies through Nordic/Mubio for distribution/resale.
- Enquire with Anje Raven for a list of polyclonal antibodies available directly from NKI.
- Transporter proteins/pharmacokinetic studies
- Stem cells
Many cell lines expressing members of the human ABC transporter pseudogene family have been created, primarily within the research groups of Piet Borst and Alfred Schinkel. Such cell lines are often used in the study of drug transport and -resistance. Please enquire for availability of such cell lines for your research at email@example.com
- Mouse models of cancer
- Knock-out mice
- Transgenic mice
genetics as a tool to unravel carcinogenesis in vivo has been a
focal point of research at NKI-AVL. Several genetically-engineered
mouse models of human
cancers (GEMMs) have been created, along with many knock-out and transgenic mice. Please enquire for availability of such for your research by sending an email to firstname.lastname@example.org.
Pattended technologies for licensing
News and events
Links naar News and Events pagina van www.nki.nl
Grants and Subsidies
People of the TTO
AT the TTO are working 50 people...
Basic Medical Statistics Course: Faculty
Dr. Michael Hauptmann received a PhD in Statistics from the University of Dortmund, Germany, in 1999. In the same year, he joined the Biostatistics Branch of the Division of Cancer Epidemiology and Genetics of the National Cancer Institute in Bethesda, Maryland, U.S.A., as a postdoctoral fellow, and became a tenure-track investigator in 2004. Since 2006, Dr. Hauptmann is a senior statistician at the Netherlands Cancer Institute in Amsterdam, The Netherlands.
Dr. Katarzyna Jozwiak obtained a Master's degree in Applied Mathematics from Delft University in 2008, and in Econometrics and Computer Science from the University of Zielona Góra, Poland, in 2009. As a graduate student in Applied Statistics at Utrecht University, she investigated optimal designs of trials with discrete-time survival endpoints and completed her PhD in 2013. After a brief period as software developer at Utrecht University, Dr. Jozwiak joined the Netherlands Cancer Institute in Amsterdam, where she is a statistical consultant for clinicians and other researchers of the Institute and the Antoni van Leeuwenhoek hospital.
Dr. Wilma Heemsbergen studied Biomedical Health Sciences at the Radboud University in Nijmegen (1988-1995). In 2008, she obtained a Ph.D. degree at the Medical Faculty of the University of Amsterdam. Her thesis was about the risks and benefits of modern conformal radiation treatment in prostate cancer. Since 1998, Dr. Heemsbergen works at the Department of Radiotherapy, and also at the Department of Epidemiology and Biostatistics since 2009. She is registered since 2008 as a senior researcher in the field of epidemiology.
Dr. Patrycja Gradowska obtained her PhD from the Department of Applied Mathematics of Delft University of Technology in 2013. For her dissertation, she developed mathematical methods and tools for assessing and managing human health benefits and risks from chemicals in food. She then became a postdoctoral researcher at the Netherlands Cancer Institute evaluating radiation-related risk of cancer following pediatric computed tomography examinations within the EPI-CT consortium. As part of her work, Dr. Gradowska also provides statistical advice to doctors and other researchers in the Institute and the Antoni van Leeuwenhoek hospital.
Apply for registration by submitting your CV and a brief description of what you expect from the course and what type of data you plan to analyze in the future to Patty Lagerweij at email@example.com. Please also include in your application whether or not
- you want to participate in the half-day "Introduction to SPSS",
- you are able to bring your own laptop,
- you are a Ph.D. student of the OOA (Onderzoeksschool Oncologie Amsterdam) or an employee of the NKI-AvL.
If you are a member of the OOA, you can also register via the registration form on the OOA website.For administrative questions, call Patty at 020-5126973. For questions related to the content of the course, you can contact Michael Hauptmann at 020-5121047.
The course is free of charge for employees of the NKI-AvL and for Ph.D. students of the OOA (Onderzoeksschool Oncologie Amsterdam). For all others, the fee is EUR 700 including the "Introduction to SPSS", course materials and coffee/tea. The fee does not include meals.
Basic Medical Statistics Course: Course Material
All the course materials (slides, data sets, exercise sheets,
suggested reading, etc.) can be downloaded by sessions
(S) and practicals (P). Please note that the website will be
updated regularly, and contents may slightly change. Handouts of
the most recent version of the slides will be provided before each
session, and exercise sheets will be provided before the
During sessions, the basic concepts will be presented and illustrated with examples. During the computer practicals, you will work on data analysis exercises while faculty is present to assist you and answer any questions you might have. At the end of each day, the exercise sheets on the website will be replaced by sheets with suggested answers.
Sessions will be held in the Piet Borst Auditorium (PBA) and practicals in room Z4 (next to PBA). Please bring your own laptop for the SPSS introduction (if you attend) and the practicals. All data sets on the website below (scroll down to the "Data sets" section) should have been downloaded to the laptop. For those who indicated not having a laptop, we will provide one for the practicals.
We recommend preparing for the course by reading the papers accompanying some of the data sets (scroll down to the bottom), as well as papers or book chapters provided under ''Suppl. Material''.
Click here to find the data sets used during sessions and practicals.
The Supllement materials can be useful resources for further reading. The list will be updated before the start of the next course.
Click here for the General overview.