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Phd Student or Postdoc telomere replication

Your function within the department

The research group of Jacqueline Jacobs at the division of Oncogenomics aims to understand the processes that act to safeguard genome integrity but that, when working inappropriately, cause cells to die, get derailed and cancerous, or become dysfunctional and contribute to aging.

More specifically, we aim to identify new factors and molecular mechanisms that are critical in telomere and genome maintenance, DNA damage responses and DNA repair.
Our projects are in large part driven by functional genetic screening in mammalian cells using shRNA and CRISPR tools, followed by in-depth mechanistic studies of selected screen hits and complemented with candidate-approaches.

In this particular project we seek to achieve an increased mechanistic understanding of telomere replication. Hereto we will combine a candidate gene approach with an unbiased functional genetic screening approach aimed at revealing novel factors and mechanisms critical in facilitating replication through telomeres. In addition we aim to understand how defects in the activity of these factors affect cell viability, growth and transformation. As aberrant telomere replication plays a key role in cancer development and progression we expect that our findings will contribute to the development of novel diagnostic approaches and therapeutic strategies for cancer.

 

Your profile

We seek to recruit an ambitious postdoc or PhD student, capable of independent thinking, with a strong commitment to science and solid experience in molecular biology and cell biology. We expect the candidate to be highly self-motivated, creative and able to work in a team.
Previous experience in the fields of DNA replication, DNA damage, DNA repair, telomeres or functional genetic screening, is a plus but not an absolute requirement.
For the postdoc experience with publication in international peer-reviewed journals is a plus.
 

Your career opportunities and terms of employment

You will join an international research team in a stimulating and interactive research environment. You will have access to state-of-the-art equipment and facilities with dedicated support staff and you will have the opportunity to follow high-quality courses offered by the NKI postdoc career development program. In case of a PhD position you will participate in the educational program of the Oncology Graduate School (OOA) Amsterdam.
We offer a competitive salary (including possibilities for additional tax-reduction) and, if necessary at the beginning, temporary housing facilities in the vicinity of the institute. For a postdoc the appointment will be for 3 years, the gross salary per month will be from € 3.215 to € 3.917 according to the FWG-function group 55, depending on education and previous experience. For a PhD student the appointment will be for 4 years, the gross salary per month will be from € 2.665 to € 3.282, according to the standard PhD scales.
The terms of employment will be in accordance with the CAO Ziekenhuizen (Collective Labour Agreement for Hospitals).


 

Interested?

For further information please visit our home page: (https://www.nki.nl/divisions/oncogenomics/jacobs-j-group/) or directly contact Dr. Jacqueline Jacobs at tel. +31 20 5121784 or e-mail: j.jacobs@nki.nl.
When applying please include a list of your publications and the contact details of 2 referees.

Selected reading:
Dev H, Chiang TW, Lescale C, de Krijger I, Martin AG, Pilger D, Coates J, Sczaniecka-Clift M, Wenming Wei, Ostermaier M, Herzog M, Lam J, Shea A, Demir M, Wu Q, Yang F, Fu B, Lai Z, Balmus G, Belotserkovskaya R, Serra V, O’Connor MJ, Bruna A, Beli P, Pellegrini L, Caldas C, Deriano L*, Jacobs JJL*, Galanty Y** and Jackson SP* Shieldin complex promotes DNA end-joining and counters homologous recombination in BRCA1-null cells. Nature Cell Biol. 20, 954–965 (2018)
*co-corresponding authors

Simonetta M, de Krijger I, Serrat J, Moatti N, Fortunato D, Hoekman L, Bleijerveld OB, Altelaar AFM, Jacobs JJL. H4K20me2 distinguishes pre-replicative from post-replicative chromatin to appropriately direct DNA repair pathway choice by 53BP1-RIF1-MAD2L2. Cell Cycle 17:1, 124-136 (2018).

Boersma V, Moatti N, Segura-Bayona S, Peuscher MH, van der Torre J, Wevers BA, Orthwein A, Durocher D and Jacobs JJL. MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end- resection. Nature 521, 537-540 (2015).

Peuscher, MH and Jacobs, JJL. DNA-damage response and repair activities at uncapped telomeres depend on RNF8. Nature Cell Biol. 13, 1139-1145 (2011).

Acquisition for this vacancy is not appreciated.