Defining the functional T cell:Tumor interactome
The Netherlands Cancer Institute (NKI)
Located in Amsterdam, NKI is an internationally renowned institute dedicated to fundamental, translational and clinical cancer research, and the only comprehensive cancer center in The Netherlands. It is a dynamic institute accommodating over 50 research groups and many advanced core facilities, including flow cytometry, proteomics, genomics, biobank and a mouse cancer center.
A functional immune defense against cancer depends on many parameters, including specific physical and dynamic contacts between cytotoxic CD8+ T cells and tumor cells, triggering numerous signaling events between and within these cell types. Whereas disruptions in T cell:tumor cell interactions contribute to immune evasion and cancer, pharmacologic intervention of interactions like PD-1:PD-L1 has significant clinical benefit. Interactions between CD8+ T cells and other immune cells have been well-characterized, but we lack a detailed map of functional interactions between CD8+ T cells and tumor cells. We hypothesize that high-resolution mapping and functional dissection of the T cell:tumor cell interactome will both provide new insight into the underlying mechanism and signaling events and identify novel pharmacologic targets to improve immunotherapy.
Together with co-PIs Karin de Visser (in vivo tumor suppression by immune cells, NKI) and Maarten Altelaar (proteomics, Utrecht), we recently received a 3M€ NWO-XL grant to study these questions. We have already established several mouse and human T cell:tumor cell co-cultures and humanized and syngeneic mouse models, which will be applied and expanded.
An ambitious technician with mouse model experience.
We seek an enthusiastic and accurate animal technician. It is strictly necessary that you hold a license for working with laboratory animals (art. 6/9/12). You should also have expertise in immunology and/or molecular biology. You will be responsible for carrying out animal experiments in collaboration with researchers on this project. Furthermore, you will contribute to wet-lab experiments and contribute to some lab management tasks. Proficiency in English and good communicative skills are important requirements, as is a team player mentality.
The vacancy is available this Fall. You will be employed for a minimum period of 2 years, with the possibility for extension. The gross salary per month can range from € 2.554,- to € 3.468,- (salary scale 45 according to the Collective Labour Agreement General Hospitals based on proven qualifications). The terms of employment will be in accordance with the CAO Hospitals and includes an end-of-year bonus and holiday pay. Acquisition for this vacancy is not appreciated.Interested?
For further information and addresses visit our website https://www.nki.nl/research/research-groups/daniel-peeper/
. You can apply via Vacancies | Netherlands Cancer Institute (nki.nl)
.The NKI values diversity and is committed to creating an inclusive work environment that stimulates the best in each individual. Applications of all individuals are welcomed regardless of age, ancestry, religion, disability, distance from the labor market, sexual orientation or gender identity.Select reading- Zhang et al, RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells, Cell Reports Medicine 2022- Apriamashvili et al, Ubiquitin ligase STUB1 destabilizes IFNγ-receptor complex to suppress tumor IFNγ signaling, Nature Comm 2022- Vredevoogd et al, The (re)discovery of tumor-intrinsic determinants of immune sensitivity by functional genetic screens, IOTech 2022- Krijgsman et al, Predictive immune-checkpoint blockade classifiers identify tumors responding to inhibition of PD-1 and/or CTLA-4, Clin Cancer Res 2021- Vredevoogd et al., Augmenting Immunotherapy Impact by Lowering Tumor TNF Cytotoxicity Threshold, Cell 2019